Generator

Part:BBa_K318024:Design

Designed by: Nathaniel Pantalone, Justin Vrana   Group: iGEM10_Wisconsin-Madison   (2010-07-13)


pLac + RBS + Int (P22) + T


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 498
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

A terminator was placed downstream of the Int encoding sequence to end transcription after the integrase is produced. Note that to fully repress the pLac promoter, overproduction of the lacI repressor protein is necessary.


Source

biobricks

References